Prestained Protein Ladder Western Blot: A Comprehensive Overview

The Prime-Step™ Prestained Broad Range Protein Ladder is a versatile tool used in western blotting to visualize protein samples on a membrane. This three-color protein standard contains 10 pre-stained proteins with molecular weights ranging from 6.5 kDa to 270 kDa, providing a broad range of molecular weight markers for easy identification and comparison.

The Prestained Broad Range Protein Ladder is characterized by its unique blue-staining bands, which are evenly spaced across the ladder. These bands are easily visible after separation on Tris-glycine gels, making it an ideal tool for protein sizing and verification. Additionally, the presence of three reference bands at 30 kDa, 52 kDa, and 270 kDa provides further confirmation of protein migration.

The Prime-Step™ Prestained Broad Range Protein Ladder has been tested on various gel running systems, including Tris-glycine gels with different concentrations (4-20%, 14%), Bis-Tris gels with MES or MOPS running buffers (4-12%), and even Tricine gels. This versatility ensures that researchers can easily adapt the ladder to their specific experimental conditions.

To demonstrate its effectiveness, a comparison was made between the Prime-Step™ Prestained Broad Range Protein Ladder and two competitor protein ladders. The results showed that the Prime-Step™ Prestained Broad Range Protein Ladder provided clearer and more consistent protein bands than the competitors, making it an excellent choice for researchers seeking reliable and accurate protein sizing.

In conclusion, the Prime-Step™ Prestained Broad Range Protein Ladder is a valuable tool for western blotting applications. Its unique three-color design and broad range of molecular weight markers make it an ideal choice for researchers who require easy identification and comparison of protein samples. With its versatility and reliability, this protein ladder has become a staple in many research laboratories worldwide.

Prestained protein ladder western blot